Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improve...

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Veröffentlicht in:Bulletin of the Korean Chemical Society 2010, Vol.31 (6), p.1568-1572
Hauptverfasser: Lee, Yeon-Ji, Park, Jong-Moon, Huh, Ji-Young, Kim, Min-Sik, Lee, Je-Sun, Palani, Arudra, Lee, Kwang-Yeol, Lee, Sang-Won
Format: Artikel
Sprache:kor
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Zusammenfassung:The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.
ISSN:0253-2964
1229-5949