제초제 quizalofop - ethyl 분석법에 관한 연구

종래에 사용해 오던 HPLC에 의한 quizalofop-ethyl의 분석법을 개선시키고, 또한 ELISA와 GLC를 이용한 새로운 quizalofop-ethyl분석법을 개발하고자 시험을 수행한 결과는 다음과 같다. ELISA 실험에서 적정배양온도는 37℃였으며 배양시간은 4시간이었다. Coating antigen의 적정수준은 20㎕/㎖이며 검출 한계는 5ppb이고 회수율은 95%이상이었다. 또 GLC와 HPLC에 의한 검출한계는 5ppb, 100ppb였으며, 토양에서의 회수율은 95.4, 89.5% 이상으로 나타났다. 이상에서 보는 바와 같이 ELISA와 GLC에 의한 quizalofop-ethyl분석법이 HPLC에 의한 것보다 양호한 것으로 나타났다. These studies were conducted to develope analysis method of herbicide quizalofop-ethyl by Gas Liquid Chromatography(GLC) and Enzyme-Linked Immunosoment Assay(ELISA) in soil and plant. Quizalofop produced by hydrolysis of quizalofop-ethyl was conjugated with bovine serum albumin(BSA). Quizalofop antibody was developed in rabbits by using BSA conjugation. Antibody titer, incubation temperature, and incubation time was 32,000, 37℃ and 4hours respectively. Minimum detection limit of quizalofop-ethyl by ELISA was 5ppb. Quizalofop-ethyl recovery from soil by ELISA was more than 95percent. Minimum detection limit of quizalofop-ethyl by GLC was 5ppb. Quizalofop-ethyl recovery from soil by GLC was from 89 percent to 100 percent. Minimun detection limit of quizalofop-ethyl by HPLC was 100ppb. Quizalofop-ethyl recovery from soil by HPLC was 89.6 percent.