항암제인 Cyclophosphamide의 중간체인 $^{15}N-Isophosphoramide$ Mustard에 관한 연구

The each nitrogen site of ifosfamide metabolite isophosphoramide mustard was synthesized with isotope enriched nitrogen. $Gylcine-^{15}N$ was converted to $2-chloroethylamine-^{15}N$ hydrochloride which was then reacted with phenyl dichlorophosphate to provide $N,N'-bis(2-chloroethyl)phosphordiamidic-^{15}N_2$ acid phenylester(50%, $PhO(O)^{15}N(CH_2CH_2Cl_2)$. Catalytic hydrogenation of this phenyl ester followed by the addition of cyclohexylamine (CHA) provided $IPM-^{15}N$ as the CHA salt(70%).