A Study of the Parameters of the Retention of Monosubstituted Benzenes in Reversed-Phase Liquid Chromatography

The relationship between the solute retention and physical parameters describing the interaction between the solute and mobile phase was investigated to predict the solute retention easily in RPLC. The retention data of monosubstituted benzenes were measured on the $\mu$-Bondapak C18 and phenyl columns with methanol-water systems. The linear relationship between dielectric increment($\epdilon'$) and retention data was observed. When the solute form hydrogen bonding with solvent molecules, the slope of the ln k' vs. $\epdilon'$ plot is changed as the compositions is varied. The quadric relationship between mixed solvent solubility parameter ($\delta$M) and retention data was observed.