무당개구리의 복피 Carotenoid 색소에 관한 연구 (제1보). ${\beta}$-Carotene과 4-Hydroxy-echinenone의 분리 및 확인

More than ten cartenoid pigments were separated from the dark red extracts of abdominal skin of Bombina Orientalis by TLC, and column chromatography, and the visible and infrared spectrum of purified carotenoids were determined. Two major pigments were identified as ${\beta}$-carotene and 4-hydroxy-echinenone respectively through physical and chemical properties, chromatographic, visible and infrared spectral characteristics. Bombina Orientalis(무당개구리)의 복피로부터 암적색의 색소를 추출하여 TLC 및 column chromatography 로 10여종의 carotenoid를 분리하여 visible 및 IR-spectrum을 조사하였다. 물리, 화학적 성질, chromatomatographic 성질 visible 및 IR-spectral 특성을 이용하여 주색소는 ${\beta}$-carotene과 4-hydroxy-echinenone임을 확인하였다.