인삼 개갑종자의 초저온동결보존을 위한 최적 건조조건과 수분함량

This study was conducted to establish the efficient protocols of the germination and cryopreservation of dehisced Korean ginseng seeds for long-term germplasm conservation. GA 3 and BA treated on seed for 24 hr facilitated germination at 5 and 10 °C. Germination percentage of desiccated seeds was decreased under moisture content (MC) of below 7.2%. Dehisced ginseng seeds were dried under airflow of laminar floor cabinet and seed drying room. The high levels (more than 90%) of germination after cryogenic exposure were obtained after drying under vertical airflow of laminar floor for 12-30 hours (MC 10.6-7.2%). Decrease in germination percentage of ginseng seeds due to desiccation damage and freezing injury was observed at MC of below 7.2% and of above 12.1%, respectively. Therefore, MC of ginseng seeds need to be controlled with a range of 8∼10% to avoid damages from both desiccation and freezing.