근적외선분광분석법 (근적외선분광분석법) 에 의한 등숙시기별 보리종실의 성분측정

This study was conducted to establish the rapid determination method for major components of maturing covered barley grains, and to improve the efficiency of selection in barley breeding. Near Infrared Reflectance Spectroscopy (NIRS) is an established, economical and nondestructive technique applied widely to the food and feed industry. 34 barley lines were sampled at 5 day-interval from 25 to 35 days after heading. A standard regression analysis for the data obtained by analytical laboratory methods and NIRS method was carved out to get a useful calibration equation. The simple significant correlation between these two methods at 25 days after heading was recognized in starch and β-glucan contents. At 30 days after heading the data obtained by two methods showed significant correlation in starch, β-glucan and protein contents. Analyzed data and that from NIRS method at 35 days after heading was significantly correlated in starch and protein contents. It was concluded that the applicability of NIRS method for the components analysis in maturing barley grains was different depending on maturing stages and components.