누에 RAPD - PCR 분석을 위한 기초연구

누에 RAPD - PCR 분석을 위한 기초연구

Reproducible the random amplified polymorphic DNAs(RAPDS) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200 μM), MgCl2,(1.5 mM) and template DNA(30 ng). In addition, anealing temperature ranging 35°C to 42°...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:한국잠사곤충학회지 1996-06, Vol.38 (1), p.7
Hauptverfasser: 황재삼, Jae Sam Hwang, 이진성, Jin Sung Lee, 이상몽, Sang Mong Lee, 강현아, Hyun Ah Kang, 황석조, Seok Jo Hwang, 서동상, Dong Sang Suh
Format: Artikel
Sprache:kor
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 1
container_start_page 7
container_title 한국잠사곤충학회지
container_volume 38
creator 황재삼
Jae Sam Hwang
이진성
Jin Sung Lee
이상몽
Sang Mong Lee
강현아
Hyun Ah Kang
황석조
Seok Jo Hwang
서동상
Dong Sang Suh
description Reproducible the random amplified polymorphic DNAs(RAPDS) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200 μM), MgCl2,(1.5 mM) and template DNA(30 ng). In addition, anealing temperature ranging 35°C to 42°C by the adjusted condition was investigated and fixed at 35°C in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorphisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorphims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDS detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori.
format Article
fullrecord <record><control><sourceid>kiss</sourceid><recordid>TN_cdi_kiss_primary_1949770</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><kiss_id>1949770</kiss_id><sourcerecordid>1949770</sourcerecordid><originalsourceid>FETCH-kiss_primary_19497703</originalsourceid><addsrcrecordid>eNpjYeA0MjI20bUwNDfhYOAtLs5MMjA1NjY3NzEw5mTQf93R8mb6BIUgxwAXBV2FAOcghdfbWt60zH0zt0XhzZyWt1PnKLzaseHNlo430ze82rqGh4E1LTGnOJUXSnMzSLu5hjh76GZnFhfHFxRl5iYWVcYbWppYmpsbGOOXBQBL5TYH</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>누에 RAPD - PCR 분석을 위한 기초연구</title><source>KoreaScience</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>황재삼 ; Jae Sam Hwang ; 이진성 ; Jin Sung Lee ; 이상몽 ; Sang Mong Lee ; 강현아 ; Hyun Ah Kang ; 황석조 ; Seok Jo Hwang ; 서동상 ; Dong Sang Suh</creator><creatorcontrib>황재삼 ; Jae Sam Hwang ; 이진성 ; Jin Sung Lee ; 이상몽 ; Sang Mong Lee ; 강현아 ; Hyun Ah Kang ; 황석조 ; Seok Jo Hwang ; 서동상 ; Dong Sang Suh</creatorcontrib><description>Reproducible the random amplified polymorphic DNAs(RAPDS) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200 μM), MgCl2,(1.5 mM) and template DNA(30 ng). In addition, anealing temperature ranging 35°C to 42°C by the adjusted condition was investigated and fixed at 35°C in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorphisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorphims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDS detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori.</description><identifier>ISSN: 2234-8174</identifier><language>kor</language><publisher>한국잠사학회</publisher><ispartof>한국잠사곤충학회지, 1996-06, Vol.38 (1), p.7</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>황재삼</creatorcontrib><creatorcontrib>Jae Sam Hwang</creatorcontrib><creatorcontrib>이진성</creatorcontrib><creatorcontrib>Jin Sung Lee</creatorcontrib><creatorcontrib>이상몽</creatorcontrib><creatorcontrib>Sang Mong Lee</creatorcontrib><creatorcontrib>강현아</creatorcontrib><creatorcontrib>Hyun Ah Kang</creatorcontrib><creatorcontrib>황석조</creatorcontrib><creatorcontrib>Seok Jo Hwang</creatorcontrib><creatorcontrib>서동상</creatorcontrib><creatorcontrib>Dong Sang Suh</creatorcontrib><title>누에 RAPD - PCR 분석을 위한 기초연구</title><title>한국잠사곤충학회지</title><addtitle>한국잠사곤충학회지</addtitle><description>Reproducible the random amplified polymorphic DNAs(RAPDS) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200 μM), MgCl2,(1.5 mM) and template DNA(30 ng). In addition, anealing temperature ranging 35°C to 42°C by the adjusted condition was investigated and fixed at 35°C in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorphisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorphims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDS detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori.</description><issn>2234-8174</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpjYeA0MjI20bUwNDfhYOAtLs5MMjA1NjY3NzEw5mTQf93R8mb6BIUgxwAXBV2FAOcghdfbWt60zH0zt0XhzZyWt1PnKLzaseHNlo430ze82rqGh4E1LTGnOJUXSnMzSLu5hjh76GZnFhfHFxRl5iYWVcYbWppYmpsbGOOXBQBL5TYH</recordid><startdate>19960630</startdate><enddate>19960630</enddate><creator>황재삼</creator><creator>Jae Sam Hwang</creator><creator>이진성</creator><creator>Jin Sung Lee</creator><creator>이상몽</creator><creator>Sang Mong Lee</creator><creator>강현아</creator><creator>Hyun Ah Kang</creator><creator>황석조</creator><creator>Seok Jo Hwang</creator><creator>서동상</creator><creator>Dong Sang Suh</creator><general>한국잠사학회</general><scope>HZB</scope><scope>Q5X</scope></search><sort><creationdate>19960630</creationdate><title>누에 RAPD - PCR 분석을 위한 기초연구</title><author>황재삼 ; Jae Sam Hwang ; 이진성 ; Jin Sung Lee ; 이상몽 ; Sang Mong Lee ; 강현아 ; Hyun Ah Kang ; 황석조 ; Seok Jo Hwang ; 서동상 ; Dong Sang Suh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kiss_primary_19497703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>1996</creationdate><toplevel>online_resources</toplevel><creatorcontrib>황재삼</creatorcontrib><creatorcontrib>Jae Sam Hwang</creatorcontrib><creatorcontrib>이진성</creatorcontrib><creatorcontrib>Jin Sung Lee</creatorcontrib><creatorcontrib>이상몽</creatorcontrib><creatorcontrib>Sang Mong Lee</creatorcontrib><creatorcontrib>강현아</creatorcontrib><creatorcontrib>Hyun Ah Kang</creatorcontrib><creatorcontrib>황석조</creatorcontrib><creatorcontrib>Seok Jo Hwang</creatorcontrib><creatorcontrib>서동상</creatorcontrib><creatorcontrib>Dong Sang Suh</creatorcontrib><collection>Korean Studies Information Service System (KISS)</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><jtitle>한국잠사곤충학회지</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>황재삼</au><au>Jae Sam Hwang</au><au>이진성</au><au>Jin Sung Lee</au><au>이상몽</au><au>Sang Mong Lee</au><au>강현아</au><au>Hyun Ah Kang</au><au>황석조</au><au>Seok Jo Hwang</au><au>서동상</au><au>Dong Sang Suh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>누에 RAPD - PCR 분석을 위한 기초연구</atitle><jtitle>한국잠사곤충학회지</jtitle><addtitle>한국잠사곤충학회지</addtitle><date>1996-06-30</date><risdate>1996</risdate><volume>38</volume><issue>1</issue><spage>7</spage><pages>7-</pages><issn>2234-8174</issn><abstract>Reproducible the random amplified polymorphic DNAs(RAPDS) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200 μM), MgCl2,(1.5 mM) and template DNA(30 ng). In addition, anealing temperature ranging 35°C to 42°C by the adjusted condition was investigated and fixed at 35°C in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorphisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorphims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDS detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori.</abstract><pub>한국잠사학회</pub><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 2234-8174
ispartof 한국잠사곤충학회지, 1996-06, Vol.38 (1), p.7
issn 2234-8174
language kor
recordid cdi_kiss_primary_1949770
source KoreaScience; EZB-FREE-00999 freely available EZB journals
title 누에 RAPD - PCR 분석을 위한 기초연구
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T10%3A41%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-kiss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%EB%88%84%EC%97%90%20RAPD%20-%20PCR%20%EB%B6%84%EC%84%9D%EC%9D%84%20%EC%9C%84%ED%95%9C%20%EA%B8%B0%EC%B4%88%EC%97%B0%EA%B5%AC&rft.jtitle=%ED%95%9C%EA%B5%AD%EC%9E%A0%EC%82%AC%EA%B3%A4%EC%B6%A9%ED%95%99%ED%9A%8C%EC%A7%80&rft.au=%ED%99%A9%EC%9E%AC%EC%82%BC&rft.date=1996-06-30&rft.volume=38&rft.issue=1&rft.spage=7&rft.pages=7-&rft.issn=2234-8174&rft_id=info:doi/&rft_dat=%3Ckiss%3E1949770%3C/kiss%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_kiss_id=1949770&rfr_iscdi=true