누에 RAPD - PCR 분석을 위한 기초연구

Reproducible the random amplified polymorphic DNAs(RAPDS) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200 μM), MgCl2,(1.5 mM) and template DNA(30 ng). In addition, anealing temperature ranging 35°C to 42°C by the adjusted condition was investigated and fixed at 35°C in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorphisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorphims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDS detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori.