형질전환체 , Bacillus thuringiensis PT0529 내에서 세가지 내독소 단백질 유전자들의 발현 특성

To characterize expression and formation of three type crystal proteins in transformant, Bacillus thuringiensis PT0529 was analysed by transmission electron microscope and SDSPAGE according to growth. The results showed that the introduced crystal protein genes, cryIVD and cytA, were well expressed at earlier stage than resident crystal protein gene of B. thuringiensis NT0423, and the formation of three-type crystal proteins were also expressed with their own morphology. However, resident crystal protein of B. thuringiensis PT0529 was smaller than that of wild type B. thuringiensis NT0423, suggesting that resident crystal protein production was interfered with introduced two type crystal protein genes.