인슐린비의존성 당뇨쥐에서 심방이뇨호르몬 농도와 mRNA 변동

Background: Fatty acid binding protein(FABP), putative mammalian fatty acid transporter, plays a role in fatty acid transport, the modulation of cellular signal transduction pathways and the protection against detergent like effects of fatty acids. FABP found in liver, adipose tissue, heart, skeletal muscle and FABP in skeletal muscle accounts for 2% of total protein mass. FABP expression has shown to be up-regulated by PPARγ in liver and adipocyte. Adipocyte and liver FABP genes have a functional PPRE(PPARγ responsive element) in their promoter region. This evidence led us to investigate for a possible the regulation of mFABP expression by PPAR in cultured human skeletal muscle cell. Methods: Myoblast were cultured in SkGM for 4weeks and were differentiated into myocyte in MEM for 4days. The myocytes were treated with PPARγ ligand(troglitazone: 5 g/mL) or transduction with adenovirus-PPARγ 1(Ad-PPAR 1). mFABP expression was identified by northern blot. Results: mFABP expression was up-regulated by 4.0±1.2 fold in the PPARγ ligand(p