파터 팽대부 선종 및 선암종에서 p73 유전자의 변이

Background/Aims: Adenoma and adenocarcinoma of the ampulla of Vater are frequently associated. We investigated to see whether p73 gene could play a role as a potential tumor suppressor gene in this adenoma-carcinoma sequence in the ampulla of Vater. Methods: Tissues of 31 ampulla of Vater cancers, 24 adenomas, 10 metastatic lymph nodes, and 35 normal regions were collected and microdissected under a microscope. DNA was extracted from the tissues and mutations in exon 5-8 of p73 gene were detected by polymerase chain reaction-single strand conformation polymorphism and direct sequencing. Results: Four base substitutions were detected in p73 gene analysis. One mutation was observed in the intron (C to T) region of one metastatic lymph node. Two mutations observed in adenocarcinoma portion of two patients showed no amino acid substitution. One mutation showed amino acid substitution (Alanine to Threonine) at codon 156 in adenoma. Conclusions: The mutations of p73 gene are rare in both adenoma and carcinoma of the ampulla of Vater. It is unclear whether p73 gene plays a role as a tumor suppressor gene.