Helicobacter pylori 감염 진단에서의 직접 연쇄중합반응법 ( Direct Polymerase Chain Reaction ) 의 역할

Background/Aims: A variety of diagnostic procedures are available for the identification of Helicobacter pylori in clinical sarnples. Recently, the detection of H. pylori in gastric biopsy samples was attempted using polymerase chain reaction, in which primers based on the sequence of a species spec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Korean journal of gastroenterology 1998-01, Vol.31 (3), p.290
Hauptverfasser: 노임환, Im Hwan Roe, 진영주, Young Joo Chin, 이대준, Dae John Lee, 이문숙, Moon Sook Lee, 송일한, Il Han Song, 임창영, Chang Yong Lim, 김정원, Jung Won Kim, 양미라, Mi Ra Yang, 김정택, Jung Taik Kim, 서은옥, Eun Ok Seo, 신지현, Ji Hyun Shin, 이종화, Jong Hwa Lee
Format: Artikel
Sprache:kor
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Background/Aims: A variety of diagnostic procedures are available for the identification of Helicobacter pylori in clinical sarnples. Recently, the detection of H. pylori in gastric biopsy samples was attempted using polymerase chain reaction, in which primers based on the sequence of a species specific antigen of H. pylori were used. The aim of this study is to test whether the PCR is useful for the detection of H. pylori. Methods: Four hundred forty gastric biopsy samples were tested. Urease test was performed and then PCR using the primer of 298 bp sized 26 kDa antigen was taken in the same 2 pieces of gastric biopsy samples. Results: Total concordance rate between the urease test and direct PCR test was 85.9%. The concordance rate between the two tests were 80.2%, 88.5% and 88.8% in gastric cancer (n=86), gastric ulcer (n=78) and duodenal ulcer (n=161) respectively. The positive rates of the urease test and the direct PCR test were 36.0% and 44.2%, respectively in the gastric cancer, 56.4% and 55.1%, in the gastric ulcer, and 84.5% and 82.0% in the duodenal ulcer. The positive rate of H. pylori infection in the gastric cancer was lower than those of the other gastroduodenal diseases. conclusions: Tbe PCR can detect H. pylori successfully in gastric biopsies using tbe 26 kDa antigen gene. The PCR technique have potential benefits of higher sensitivity and quicker detection than the urease test. Further study is necessary to establish PCR test as a diagnostic method of H. pylori and to elucidate the characteristics of H. pylori. (Korean J Gastroenterol 1998;31:290 - 297)
ISSN:1598-9992