자궁경부종양침윤 임파구와 종양주위 임파절 임파구의 면역학적 특성

To evaluate the immuniligical characteristics of turnnor infiltrating lymphocytes(TIL) and autologous peripheral blood lyrnphcytes(A-PBL) in 20 patient.', with primary cervical tuMors and tumor draining lyrnph node lymphortypes from 10 of thern, lymphocytes were cultured in vitro for proliferation, phenotyping and cytotoxicity. Fredhly obtained cervical tumor masses and draining lymph nodes were digersted enzymatically. Lymphocytes were sepatated using ficoll-hypaque density gradient method, and were cultured in Waymouth's MB 752/1 media with 5% human AB serum only or with 1,000U/ml recombinant interleukin-2(rlL-2). The resultsl were as follows ; 1. Lymphocytes from primary cervical rumors constituted from 20% to 90% of single cell tumor suspensions ( average lymphocyte cell: tumor cell ratio = 0.95) and expanded from 2.6 - folds to 130 - folds in 12 of 20 cultures. For 10 of 20 patients, lymohccytes derived from tumor darining lymph vodes proliferated in culture from 2.8 - folds to 120-folds. 2. 2. When TIL, as well as A-PBL were cultured in 1,000U/ml of rIL-2, TIL significantly proliferated than A-PBI,(p