A polymorphic, telomeric-like sequence microsatellite in the Neotropical fish Prochilodus

A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT) n repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three P...

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Veröffentlicht in:	Cytogenetic and genome research 2002-01, Vol.98 (4), p.308-310
Hauptverfasser:	Hatanaka, T., Henrique-Silva, F., Galetti Jr, P.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Cytogenetics and Comparative Mapping Animals Biological and medical sciences Cytogenetics Fishes - genetics Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution In Situ Hybridization, Fluorescence Methods and miscellaneous Microsatellite Repeats - genetics Prochilodus Species Specificity Telomere - genetics Tropical Climate
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Zusammenfassung:	A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT) n repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three Prochilodus species. Fluorescence in situ hybridization analysis showed signals predominantly in the telomeric regions of several chromosomes. The description of this microsatellite may contribute to studies of the population structure of this fish group.
ISSN:	1424-8581 1424-859X
DOI:	10.1159/000071054