Contribution of Serotonin 3A Receptor to Motor Function and Its Expression in the Gastrointestinal Tract

Introduction: The serotonin 3A receptor (5-HT 3A R) is involved in vomiting and gastrointestinal motility. However, it is not well understood the expression pattern of 5-HT 3A R in the gut immunohistochemically and how much contribution of 5-HT 3A R to upper or lower intestinal motility. Objectives: We investigated the contribution of 5-HT 3A R to gastrointestinal motor function by using 5-HT 3A R KO mice and sought to identify 5-HT 3A R-expressing cells via immunohistochemical staining using 5-HT 3A R-GFP reporter mice. Methods: The expression of 5-HT 3A R was measured in each section of the gut through real-time PCR. The motor function of the stomach and colon was assessed via the 13 C-octanoic acid breath test and colonic bead expulsion test, respectively, using 5-HT 3A R KO mice. 5-HT 3A R-expressing cells in the muscle layer of the gut were identified by immunohistochemical staining using 5-HT 3A R-GFP reporter mice. Results: 5-HT 3A R was expressed throughout the digestive tract, and 5-HT 3A R expression in the stomach and lower digestive tract was higher than that in the other sections. Motor function in the stomach and colon was lower in 5-HT 3A R KO mice than in WT mice. As a result of immunohistochemical staining using GFP reporter mice, cholinergic neurons and PDGFRα + cells were shown to express 5-HT 3A R. In contrast, 5-HT 3A R indicated by GFP fluorescence was rarely detected in ICC and smooth muscle cells. Conclusions: These results show that 5-HT 3A R is highly expressed in the stomach and large intestine and that the activation of 5-HT 3A R accelerates gastric emptying and large intestine transit. Additionally, 5-HT 3A R is highly expressed in cholinergic neurons and some interstitial cells, such as PDGFRα + cells.