EFFECT OF NITROGEN FERTILIZER ON GRAIN OF WINTER WHEAT

A huge amount of experimental information concerning the effects of N-fertilizers and the pathways of N-transformation in ecosystems is available. The general evaluation of this, however, is hardly possible, while the ecological conditions of the experiments are different and the dynamic studies mostly deal with certain subprocess but neglecting their interactions. In this paper the quantity of amino acids proteins hydrolisable with 6 molar HCl in winter wheat grain was determined by amino acid analyser, while the quantity of free amino acids were analysed by high-performance liquid chromatography (with ophthaldialdehyde reagent) and protein content was measured by acryl-amide gelelectophoresis (PAGE). It can be stated that in the content of protein amino acids the quantity of glutamic acid and proline increased most, but in all other N-fertilised treatments the quantity of amino acids raised apparently. In case of free amino acids, primarily the content of asparagine and leucine, increased compared to the control samples due to the effect of fertiliser. All changes of the measured protein, free amino acids and proteins were significantly different from the control.