23Na Nuclear Magnetic Resonance Relaxation Studies of Sodium Ion Interaction with Soluble RNA

23Na Nuclear Magnetic Resonance Relaxation Studies of Sodium Ion Interaction with Soluble RNA

Interactions between 23Na+ and soluble RNA in aqueous solution are studied with the use of 23Na nuclear magnetic resonance. At low concentrations of NaCl, the interactions obey a simple equilibrium model with a formation constant log (Kf)s = 2.8 ± 0.3. The relaxation rate of the bound sodium is foun...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1969-03, Vol.62 (3), p.644-649
Hauptverfasser: James, Thomas L., Noggle, Joseph H.
Format: Artikel
Sprache:eng
Schlagworte:
Aqueous solutions
Electric fields
Ions
Macromolecules
Magnetic Resonance Spectroscopy
Models, Theoretical
Nuclear magnetic resonance
Physical Sciences: Chemistry
Quadrupoles
Relaxation time
RNA
RNA, Transfer
Sodium
Sodium Chloride
Sodium Isotopes
Yeasts
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Zusammenfassung:Interactions between 23Na+ and soluble RNA in aqueous solution are studied with the use of 23Na nuclear magnetic resonance. At low concentrations of NaCl, the interactions obey a simple equilibrium model with a formation constant log (Kf)s = 2.8 ± 0.3. The relaxation rate of the bound sodium is found to be T1B-1 = 222 ± 19 sec-1 compared to that of free sodium T1F-1 = 17.5 sec-1. At high NaCl concentrations, the system deviates from the model, possibly owing to aggregation of the soluble RNA.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.62.3.644