An Alternative Modality for the Immunodetection of the Molecular Phenotypes of Coagulation Factor XIIIB

An Alternative Modality for the Immunodetection of the Molecular Phenotypes of Coagulation Factor XIIIB

A new technical modality for the typing of coagulation factor XIIIB (F13B) is described. The determination of F13B phenotypes was carried out from desyalized plasma samples by means of polyacrylamide gel isoelectric focusing (pH range 6-8) followed by immunofixation-silver stain. This method combine...

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Veröffentlicht in:Human heredity 1993-07, Vol.43 (4), p.257-260
Hauptverfasser: Cameselle, F.D., Caeiro, B., Riveiro, A.
Format: Artikel
Sprache:eng
Schlagworte:
Alleles
Factor XIII - chemistry
Factor XIII - genetics
Gene Frequency
Humans
Immunochemistry
Isoelectric Focusing
Original Paper
Phenotype
Polymorphism, Genetic
Spain
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Zusammenfassung:A new technical modality for the typing of coagulation factor XIIIB (F13B) is described. The determination of F13B phenotypes was carried out from desyalized plasma samples by means of polyacrylamide gel isoelectric focusing (pH range 6-8) followed by immunofixation-silver stain. This method combines high sensitivity, low expenditure, and a single methodology. A genetic analysis on F13B phenotypes in the Galician
ISSN:0001-5652
1423-0062
DOI:10.1159/000154140