PHYLOGENETIC ANALYSIS OF NORTH AMERICAN PLUMS (PRUNUS SECT. PRUNOCERASUS: ROSACEAE) BASED ON NUCLEAR LEAFY AND S6PDH SEQUENCES
Phylogenetic analysis of DNA sequences from two low-copy nuclear genes (LEAFY and s6pdh) was conducted to investigate relationships among native plums of North America (Prunus sect. Prunocerasus). The cladograms resulting from maximum parsimony and Bayesian inference are poorly resolved and incongru...
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Veröffentlicht in: | Journal of the Botanical Research Institute of Texas 2008-07, Vol.2 (1), p.401-414 |
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Zusammenfassung: | Phylogenetic analysis of DNA sequences from two low-copy nuclear genes (LEAFY and s6pdh) was conducted to investigate relationships among native plums of North America (Prunus sect. Prunocerasus). The cladograms resulting from maximum parsimony and Bayesian inference are poorly resolved and incongruent with clades based on chloroplast DNA sequences. Evidence from both nuclear genes confirms the placement of P. texana in sect. Prunocerasus. Sequences of P. americana and P. mexicana form a monophyletic group on the s6pdh cladogram, but most P. americana collections also have a second divergent sequence, which together form a clade sister to P. subcordata. The shared possession of a 250-bp insertion within LEAFY intron 2 supports a close relationship between P. hortulana and P. murrayana. Prunus alleghaniensis, P. gracilis, P. marítima, and P. umbellata form a paraphyletic group on the LEAFY cladogram, basal to a clade composed of P. angustijolia and angustifolia-like sequences of P. rivularis and P. munsoniana. Data from LEAFY and s6pdh suggest hybrid origins for P. rivularis and P. munsoniana, with P. angustifolia as one progenitor. Se realizó un análisis filogenético de secuencias de DNA de dos genes nucleares (LEAFY y s6pdh) para investigar las relaciones entre los ciruelos nativos de Norte América (Prunus sect. Prunocerasus). Los cladogramas que resultan de la máxima parsimonia e inferencia bayesiana tienen una resolución pobre y son incongruentes con ciados basados en secuencias de DNA plastidial. Los resultados de ambos genes nucleares confirman la colocación de P. texana en la sect. Prunocerasus. Las secuencias de P. americana y P. mexicana forman un grupo monofilético en el cladograma de s6pdh, pero la mayor parte de las colecciones de P. americana tienen una segunda secuencia divergente, que juntas forman un ciado hermano de P. subcordata. La posesión compartida de una inserción de 250-bp en el intrón 2 de LEAFY apoya una relación fuerte entre P. hortulana P. murrayana. Prunus alleghaniensis, P. gracilis, P. maritima, y P. umbellata forman un grupo parafilético en el cladograma de LEAFY, basal al ciado compuesto por P angustijolia y secuencias similares a angustifolia de P. rivularis y P. munsoniana. Los datoa de LEAFY y sópdh sugieren origen híbrido para P. rivularis y P. munsoniana, con P. angustijolia como uno de los progenitores. |
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ISSN: | 1934-5259 |