The Role of RNA Polymerase σ Subunit in Promoter-Independent Initiation of Transcription
In bacteria, initiation of transcription depends on the RNA polymerase σ subunit, which brings catalytically proficient RNA polymerase core to promoters by binding to specific DNA elements located upstream of the transcription start point. Here, we study σ-dependent synthesis of a transcript that is...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2004-03, Vol.101 (13), p.4396-4400 |
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Sprache: | eng |
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Zusammenfassung: | In bacteria, initiation of transcription depends on the RNA polymerase σ subunit, which brings catalytically proficient RNA polymerase core to promoters by binding to specific DNA elements located upstream of the transcription start point. Here, we study σ-dependent synthesis of a transcript that is used to prime replication of the single-stranded genome of bacteriophage M13. We show that, in this system, σ plays no role in DNA recognition, which is accomplished solely through RNA polymerase core interaction with DNA downstream of the transcription start point. However, σ is required for full-sized transcript synthesis by allowing RNA polymerase core to escape into productive elongation. RNA polymerase σ may play a similar role during replication primer synthesis in other bacterial mobile elements whose life cycle involves a single-stranded DNA stage. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0400886101 |