Isolation and characterization of a cDNA clone for the Cat2 gene in maize and its homology with other catalases

A 230-base-pair cDNA representing the 3′ end of the Cat2 gene in maize was isolated and used to screen a λ gt11 cDNA library made from scutellar poly(A)+ RNA. Several clones were subcloned into a pUC12 vector; one of the subclones, pCat2.1c, appears to represent a near-complete sequence of the Cat2...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1987-10, Vol.84 (19), p.6830-6834
Hauptverfasser:	Bethards, L.A, Skadsen, R.W, Scandalios, J.G
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Amino Acid Sequence Amino acids Base Sequence Biological and medical sciences CATALASA CATALASE Catalase - genetics catalase-2 Cloning, Molecular Complementary DNA Corn DNA DNA - isolation & purification DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology GENE GENES Genes. Genome Genetic hybridization Genetic Vectors ISOENZIMAS ISOENZYME ISOENZYMES Liver Messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization NUCLEOTIDE nucleotide sequence NUCLEOTIDES NUCLEOTIDOS Plants - enzymology Plants - genetics Plasmids RNA Yeasts ZEA MAYS Zea mays - enzymology Zea mays - genetics
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Zusammenfassung:	A 230-base-pair cDNA representing the 3′ end of the Cat2 gene in maize was isolated and used to screen a λ gt11 cDNA library made from scutellar poly(A)+ RNA. Several clones were subcloned into a pUC12 vector; one of the subclones, pCat2.1c, appears to represent a near-complete sequence of the Cat2 coding region. Immunological screening, hybridization-selection translation, and RNA blot analysis confirmed that pCat2.1c was derived from the Cat2 transcript. The DNA sequence of pCat2.1c was determined. The deduced amino acid sequence shows homology with catalase amino acid sequences from rat liver, bovine liver, human kidney, and yeast.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.84.19.6830