Toward an Understanding of the Folding of Ribonuclease A

Toward an Understanding of the Folding of Ribonuclease A

A mechanism was proposed several years ago for the regeneration of native ribonuclease A (EC 3.1.27.5) from the fully reduced form by a mixture of oxidized and reduced glutathiones. Several folding pathways, depending on the solution conditions, were deduced. It is shown here that recent criticisms...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1987-08, Vol.84 (16), p.5740-5744
Hauptverfasser: Scheraga, Harold A., Konishi, Yasuo, Rothwarf, David M., Mui, Philip W.
Format: Artikel
Sprache:eng
Schlagworte:
Algorithms
Biochemistry
Biological and medical sciences
Biophysics
Chemical bonding
Conformational dynamics in molecular biology
Disulfides
Energy value
Free energy
Fundamental and applied biological sciences. Psychology
Kinetics
Mathematical constants
Molecular biophysics
Molecules
Protein Conformation
Protein refolding
Ribonuclease, Pancreatic
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Beschreibung
Zusammenfassung:A mechanism was proposed several years ago for the regeneration of native ribonuclease A (EC 3.1.27.5) from the fully reduced form by a mixture of oxidized and reduced glutathiones. Several folding pathways, depending on the solution conditions, were deduced. It is shown here that recent criticisms of those results are due to a misinterpretation of the analysis of our data. A more detailed description of our method of analysis of our previous kinetic and energetic data is presented in order to clarify possible misconceptions.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.16.5740