method for the spectrophotometric measurement of chlorophyll a and pheophytin a in benthic microalgae

method for the spectrophotometric measurement of chlorophyll a and pheophytin a in benthic microalgae

A method is described that allows the measurement of river periphyton chlorophyll a and pheophytin a both on rocks and in soft sediments. The method involves successive extractions directly from frozen samples with boiling methanol. Pigments are estimated by optical density measurements before and a...

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Veröffentlicht in:Limnology and oceanography 1975-01, Vol.20 (5), p.887-896
Hauptverfasser: Tett, P, Kelly, M.G, Hornberger, G.M
Format: Artikel
Sprache:eng
Schlagworte:
Acidification
Chlorophylls
Confidence limits
Optical density
Periphyton
Pigments
plant biochemistry
plant physiology
Sand
Sediments
Solvents
Wavelengths
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Zusammenfassung:A method is described that allows the measurement of river periphyton chlorophyll a and pheophytin a both on rocks and in soft sediments. The method involves successive extractions directly from frozen samples with boiling methanol. Pigments are estimated by optical density measurements before and after acidification. Methanol is superior to acetone for the distinction of chlorophyll a and pheophytin a by this means.
ISSN:0024-3590
1939-5590
DOI:10.4319/lo.1975.20.5.0887