Energy landscape in protein folding and unfolding

Energy landscape in protein folding and unfolding

We use ¹H NMR to probe the energy landscape in the protein folding and unfolding process. Using the scheme ⇄ reversible unfolded (intermediate) → irreversible unfolded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs when the temperature is increased. Using therm...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2016-03, Vol.113 (12), p.3159-3163
Hauptverfasser: Mallamace, Francesco, Corsaro, Carmelo, Mallamace, Domenico, Vasi, Sebastiano, Vasi, Cirino, Baglioni, Piero, Buldyrev, Sergey V., Chen, Sow-Hsin, Stanley, H. Eugene
Format: Artikel
Sprache:eng
Schlagworte:
BASIC BIOLOGICAL SCIENCES
Biological Sciences
Energy
energy landscape
hydration water
Hydrogen
Muramidase - chemistry
NMR
Nuclear magnetic resonance
Peptides
Physical Sciences
Protein Denaturation
Protein Folding
Proteins
Proton Magnetic Resonance Spectroscopy
proton NMR
Temperature
Water
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Zusammenfassung:We use ¹H NMR to probe the energy landscape in the protein folding and unfolding process. Using the scheme ⇄ reversible unfolded (intermediate) → irreversible unfolded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs when the temperature is increased. Using thermal cycles in the range 295
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1524864113