Purification and partial characterisation of a 60 KDa laccase from Fomitiporia mediterranea

A 60-kDa extra-cellular laccase enzyme was purified to homogeneity from a liquid culture of Fomitiporia mediterranea, the main wood rot agent of the grapevine disease esca. The enzyme exhibited thermal stability. Substrate specificity and inhibitor studies indicated that the enzyme was a typical fun...

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Veröffentlicht in:	Phytopathologia mediterranea 2009-12, Vol.48 (3), p.447-453
Hauptverfasser:	Abou-Mansour, E, Polier, J, Pezet, R, Tabacchi, R
Format:	Artikel
Sprache:	eng
Schlagworte:	chemical constituents of plants decay fungi enzyme activity enzyme inhibitors enzyme substrates esca Fomitiporia mediterranea fungal diseases of plants fungal proteins grapes laccase oxidation phenolic compounds plant pathogenic fungi polyphenols Polyporales purification RESEARCH PAPERS signs and symptoms (plants) substrate specificity thermal stability Vitis vinifera
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Zusammenfassung:	A 60-kDa extra-cellular laccase enzyme was purified to homogeneity from a liquid culture of Fomitiporia mediterranea, the main wood rot agent of the grapevine disease esca. The enzyme exhibited thermal stability. Substrate specificity and inhibitor studies indicated that the enzyme was a typical fungal laccase. Several natural phenolic and polyphenolic compounds were oxidised by the isolated laccase.
ISSN:	0031-9465 1593-2095