Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell

We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structura...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2008-09, Vol.105 (38), p.14271-14276
Hauptverfasser:	Hein, Birka, Willig, Katrin I, Hell, Stefan W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Cell Line Cell Survival Cells Cells - cytology Cellular biology Diffraction Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - ultrastructure Fluorescence Image resolution Imaging Laser beams Lasers Luminescent Proteins - genetics Luminescent Proteins - metabolism Mammals Microscopes Microscopy Microscopy, Confocal Microscopy, Fluorescence - economics Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Microscopy, Fluorescence - standards Molecules Physical Sciences Potoroidae Proteins Stimulated emission Wave diffraction
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Zusammenfassung:	We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structural elements of the ER revealed a focal plane (x, y) resolution of
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.0807705105