A stereospecific solid-phase screening assay for colonies expressing both (R)- and (S)-selective ω-aminotransferases

A stereospecific solid-phase screening assay for colonies expressing both (R)- and (S)-selective ω-aminotransferases

A novel solid-phase screening assay was developed for colonies expressing both (R)- and (S)-selective ω-aminotransferases. This high-throughput assay can be used to screen rapidly large variant libraries with enhanced substrate selectivity and enantioselectivities.

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Veröffentlicht in:Philosophical transactions of the Royal Society of London. Series A: Mathematical, physical, and engineering sciences physical, and engineering sciences, 2016-02, Vol.374 (2061), p.1-6
Hauptverfasser: Willies, Simon C., Galman, James L., Slabu, Iustina, Turner, Nicholas J.
Format: Artikel
Sprache:eng
Schlagworte:
Amines
Amino acids
Directed molecular evolution
Enzymes
Escherichia coli
Genetic screening
Ketones
Materials
Oxidases
Proteins
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Zusammenfassung:A novel solid-phase screening assay was developed for colonies expressing both (R)- and (S)-selective ω-aminotransferases. This high-throughput assay can be used to screen rapidly large variant libraries with enhanced substrate selectivity and enantioselectivities.
ISSN:1364-503X
1471-2962