Measurement of Nitrite Reductase in Leaf Tissue of Vigna mungo: A New Method

Measurement of Nitrite Reductase in Leaf Tissue of Vigna mungo: A New Method

The enzyme nitrite reductase (EC 1.6.6.4) is generally assayed in terms of disappearance of nitrite from the assay medium. We describe a technique which allowed estimation of the enzyme level in leaf tissues of Vigna mungo (L). Hepper in terms of the release of the product (NH3) of the enzyme reacti...

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Veröffentlicht in:Planta 1980-01, Vol.147 (3), p.196-198
Hauptverfasser: Srivastava, R.C., Bose, Bandana, Mukerji, D., Mathur, S.N., Srivastava, H.S.
Format: Artikel
Sprache:eng
Schlagworte:
Enzyme activity
Enzymes
Nitrites
Physiological assimilation
Solvents
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Zusammenfassung:The enzyme nitrite reductase (EC 1.6.6.4) is generally assayed in terms of disappearance of nitrite from the assay medium. We describe a technique which allowed estimation of the enzyme level in leaf tissues of Vigna mungo (L). Hepper in terms of the release of the product (NH3) of the enzyme reaction. The technique is offered as an alternative, possibly more convenient method for assay of nitrite reductase in plant tissue in vivo.
ISSN:0032-0935
1432-2048