Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization
[$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the regio...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1970-11, Vol.170 (3958), p.639-641 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 641 |
|---|---|
| container_issue | 3958 |
| container_start_page | 639 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 170 |
| creator | Wimber, D. E. Steffensen, D. M. |
| description | [$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [$^{3}$H]RNA and DNA was confined to the 56E-F segment. |
| format | Article |
| fullrecord | <record><control><sourceid>jstor</sourceid><recordid>TN_cdi_jstor_primary_1731522</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>1731522</jstor_id><sourcerecordid>1731522</sourcerecordid><originalsourceid>FETCH-jstor_primary_17315223</originalsourceid><addsrcrecordid>eNpjYuA0NLA01bU0MjBmYeA0MDA207UwMDflYOAqLs4yMADKWRpzMvj75Ccn5mRWJZZk5ucp5KcpmAYrBPk5Krgn5qUWKwCFXIryi_MLMjJzEhWcM4ryc4G8XKBMUiVIma4LUKlHZVJRZgrUCB4G1rTEnOJUXijNzSDj5hri7KGbVVySXxRfUJSZm1hUGW9obmxoamRkTEAaAOobOZA</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization</title><source>Science Magazine</source><source>Jstor Complete Legacy</source><creator>Wimber, D. E. ; Steffensen, D. M.</creator><creatorcontrib>Wimber, D. E. ; Steffensen, D. M.</creatorcontrib><description>[$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [$^{3}$H]RNA and DNA was confined to the 56E-F segment.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><language>eng</language><publisher>American Association for the Advancement of Science</publisher><subject>Chromosomes ; DNA ; Drosophila ; Electrodes ; Gels ; Genes ; Genetic hybridization ; Insect larvae ; Radioactive decay ; RNA</subject><ispartof>Science (American Association for the Advancement of Science), 1970-11, Vol.170 (3958), p.639-641</ispartof><rights>Copyright 1970 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1731522$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1731522$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,57995,58228</link.rule.ids></links><search><creatorcontrib>Wimber, D. E.</creatorcontrib><creatorcontrib>Steffensen, D. M.</creatorcontrib><title>Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization</title><title>Science (American Association for the Advancement of Science)</title><description>[$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [$^{3}$H]RNA and DNA was confined to the 56E-F segment.</description><subject>Chromosomes</subject><subject>DNA</subject><subject>Drosophila</subject><subject>Electrodes</subject><subject>Gels</subject><subject>Genes</subject><subject>Genetic hybridization</subject><subject>Insect larvae</subject><subject>Radioactive decay</subject><subject>RNA</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNpjYuA0NLA01bU0MjBmYeA0MDA207UwMDflYOAqLs4yMADKWRpzMvj75Ccn5mRWJZZk5ucp5KcpmAYrBPk5Krgn5qUWKwCFXIryi_MLMjJzEhWcM4ryc4G8XKBMUiVIma4LUKlHZVJRZgrUCB4G1rTEnOJUXijNzSDj5hri7KGbVVySXxRfUJSZm1hUGW9obmxoamRkTEAaAOobOZA</recordid><startdate>19701106</startdate><enddate>19701106</enddate><creator>Wimber, D. E.</creator><creator>Steffensen, D. M.</creator><general>American Association for the Advancement of Science</general><scope/></search><sort><creationdate>19701106</creationdate><title>Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization</title><author>Wimber, D. E. ; Steffensen, D. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-jstor_primary_17315223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Chromosomes</topic><topic>DNA</topic><topic>Drosophila</topic><topic>Electrodes</topic><topic>Gels</topic><topic>Genes</topic><topic>Genetic hybridization</topic><topic>Insect larvae</topic><topic>Radioactive decay</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wimber, D. E.</creatorcontrib><creatorcontrib>Steffensen, D. M.</creatorcontrib><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wimber, D. E.</au><au>Steffensen, D. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><date>1970-11-06</date><risdate>1970</risdate><volume>170</volume><issue>3958</issue><spage>639</spage><epage>641</epage><pages>639-641</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>[$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [$^{3}$H]RNA and DNA was confined to the 56E-F segment.</abstract><pub>American Association for the Advancement of Science</pub></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1970-11, Vol.170 (3958), p.639-641 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_jstor_primary_1731522 |
| source | Science Magazine; Jstor Complete Legacy |
| subjects | Chromosomes DNA Drosophila Electrodes Gels Genes Genetic hybridization Insect larvae Radioactive decay RNA |
| title | Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T19%3A05%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Localization%20of%205S%20RNA%20Ganes%20on%20Drosophila%20Chromosomes%20by%20RNA-DNA%20Hybridization&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Wimber,%20D.%20E.&rft.date=1970-11-06&rft.volume=170&rft.issue=3958&rft.spage=639&rft.epage=641&rft.pages=639-641&rft.issn=0036-8075&rft.eissn=1095-9203&rft_id=info:doi/&rft_dat=%3Cjstor%3E1731522%3C/jstor%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_jstor_id=1731522&rfr_iscdi=true |