Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization

Localization of 5S RNA Ganes on Drosophila Chromosomes by RNA-DNA Hybridization

[$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the regio...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1970-11, Vol.170 (3958), p.639-641
Hauptverfasser: Wimber, D. E., Steffensen, D. M.
Format: Artikel
Sprache:eng
Schlagworte:
Chromosomes
DNA
Drosophila
Electrodes
Gels
Genes
Genetic hybridization
Insect larvae
Radioactive decay
RNA
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Zusammenfassung:[$^{3}$H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [$^{3}$H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [$^{3}$H]RNA and DNA was confined to the 56E-F segment.
ISSN:0036-8075
1095-9203