Relationship between Number of Spleen Colonies and125IdUrd Incorporation into Spleen and Femur

Relationship between Number of Spleen Colonies and125IdUrd Incorporation into Spleen and Femur

Graded numbers of bone marrow (BM) cells were injected into fatally irradiated mice. Eight days later the mice were given 3.0 μ Ci (1 Ci = 3.7× 1010Bq) of125IdUrd to label proliferating cells in the spleen and BM. On day 9 the mice were killed and the spleens and femurs were removed for splenic colo...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1983-01, Vol.80 (2), p.435-438
Hauptverfasser: Inoue, T., Bullis, J. E., Cronkite, E. P., Hubner, G. E.
Format: Artikel
Sprache:eng
Schlagworte:
Bone marrow
Cell growth
Cells
Cellular differentiation
Femur
Interphase
Pluripotent stem cells
Seeding
Spleen
Stem cells
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Zusammenfassung:Graded numbers of bone marrow (BM) cells were injected into fatally irradiated mice. Eight days later the mice were given 3.0 μ Ci (1 Ci = 3.7× 1010Bq) of125IdUrd to label proliferating cells in the spleen and BM. On day 9 the mice were killed and the spleens and femurs were removed for splenic colony assay and measurement of radioactivity in the spleen and femurs. The number of splenic colonies shows a linear relationship with dose of marrow cells injected from 104to 105cells. The slope of the curve of spleen colonies versus number of cells injected is
ISSN:0027-8424