Interleukin 2 is Mitogenic for nu/nu and nu/+ Murine Spleen Cells

Interleukin 2 (IL-2), a product of activated T lymphocytes but not of T-cell-deficient spleen cells of congenitally athymic (nu/nu) mice, is shown to be strongly mitogenic for spleen cells from both nu/nu and nu/+ mice in the absence of serum. This response does not depend on the presence of additional mitogen or antigen as has been previously reported and is dose dependent with respect to IL-2. Plots of the logarithm of cell number versus logarithm of response indicate that IL-2-induced mitogenesis of nu/nu spleen cells is a one-cell event (slope = 1.28± 0.21, mean ± SD), whereas the response of nu/+ spleen cells to concanavalin A is a three-cell event (slope = 3.18± 0.16). The nu/nu spleen cells proliferating in response to IL-2 are at least 80% Tlymphocytes by the third day of culture, as demonstrated by lysis with monoclonal anti-Thy-1 antibody and complement. These results suggest that one of the major roles of the thymus may be to induce IL-2 production in a subpopulation of lymphocytes that is then persistent.