BAS/BSCR25 Importance of interaction between peroxisome proliferator-activated receptor-α and NADPH oxidases in cardiac hypertrophy

Peroxisome proliferator-activated receptor-α (PPAR-α) and NADPH oxidases are known individually to have essential roles in left ventricular hypertrophy (LVH). However, the potential importance of interaction between the two in overall regulation of the hypertrophic phenotype is unclear. Here Nox2−/−, PPAR-α−/− and matched wild-type (WT) mice (n=8) underwent thoracic aortic constriction (TAC) or sham surgery and were studied 7 days later. No differences in basal contractile function (echocardiography) or LVH were observed. However, increased mRNA expression (real-time RT-PCR) of PPAR-α in Nox2−/− (1.49%±0.08 vs WT, 1.04%±0.11 arbitrary units; p