The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonastransposon Tn501

The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr-111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the...

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Veröffentlicht in:Nucleic acids research 1985-08, Vol.13 (15), p.5657-5669
Hauptverfasser: Brown, Nigel L., Winnie, Joseph N., Fritzinger, David, Pridmore, R.David
Format: Artikel
Sprache:eng
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Zusammenfassung:The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr-111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/13.15.5657