Effect of Sampling Site and Collection Method on Variations in Baseline Clinical Pathology Parameters in Fischer-344 Rats

Effect of Sampling Site and Collection Method on Variations in Baseline Clinical Pathology Parameters in Fischer-344 Rats. II. Clinical Hematology. SMITH, C.N., NEPTUN, D. A., AND IRONS, R.D. (1986). Fundam. Appl. Toxicol. 7, 658-663. An analysis of the influence of blood-sampling site and collection method on peripheral hematology parameters was conducted in age-matched male Fischer-344 rats. Sites examined for blood collection were the right ventricle, abdominal aorta, abdominal vena cava, retroorbital plexus, and tail. Collection methods used included syringe (10 ml), Vacutainer(s) (3 ml or 3 ml × 2), and capillary tube. Blood collected from the tail exhibited leukocyte counts approximately twice those of samples from other sites. Blood collected from the retroorbital plexus and tail exhibited significant variations in white blood cell count, red blood cell count, hemoglobin, and hematocrit, and differences in leukocyte differential counts of lymphocytes and neutrophils when compared with other sites. Blood collected from the abdominal aorta and in a second Vacutainer from the right ventricle exhibited lower erythrocyte, leukocyte, and platelet counts than that collected from other sites with the exception of the platelet count from tail blood which was lower than that from all other sites. Although parameter values vary with sample site selection, those obtained from right ventricle blood were the least variable and the most consistent when compared with all other methods.