Neuropeptide Y Detection Using Aptamer-Modified Electrodes By Electrochemical Impedance Spectroscopy

The advantages of using carbon fiber, gold or platinum as electrodes are because they are promising materials with high electrical conductivity, chemical stability, and high surface area that can be easily modified on their surface. The immobilization and biofouling of the surface of the electrodes were studied and compared using Electrochemical Impedance Spectroscopy (EIS). Adsorption of NPY to the aptamer-modified microelectrodes was also demonstrated by EIS with the specificity provided by aptamer functionalization of the electrode surface. Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Atomic Force Microscopy (AFM) was used to characterize the morphology, the elemental composition, and the topography of the electrodes before and after the aptamer modification, respectively. NPY concentrations from the range of pg/mL to ng/mL were detected to the see the interaction between the modified electrodes and our target. These results give us a great approach of measure NPY using EIS.