A Label-Free Molecularly Imprinted Electrochemical Sensor Based on MXene Nanosheets Modified by Gold Nanoparticles for Sensitive and Selective Detection of Homocysteine
A label-free molecularly imprinted electrochemical sensor (MIECS) based on electropolymerized molecularly imprinted polymer (MIP) was developed for the determination of homocysteine (Hcy) in serum for the first time. MXene@AuNPs with layered structure was synthesized on the electrode by modifying MX...
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Veröffentlicht in: | Journal of the Electrochemical Society 2022-08, Vol.169 (8), p.87503 |
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Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A label-free molecularly imprinted electrochemical sensor (MIECS) based on electropolymerized molecularly imprinted polymer (MIP) was developed for the determination of homocysteine (Hcy) in serum for the first time. MXene@AuNPs with layered structure was synthesized on the electrode by modifying MXene with gold nanoparticles (AuNPs). MIP based on dopamine hydrochloride (DA) were electropolymerized onto the surface of MXene@AuNPs modified electrode by molecular docking and quantum chemical calculations for specific recognition of Hcy. MXene@AuNPs as a carrier for immobilizing MIP steadily enhances the conductivity of the electrode (about 4.2-fold) and plays a crucial part in improving the detection sensitivity of MIECS. The results showed that the current response linearly decreased with the increasing concentration of Hcy in the detection range from 1 × 10
−13
to 1 × 10
−5
mol l
−1
, the limit of detection (LOD) and limit of quantification (LOQ) of 11.81 fmol l
−1
and 39.49 fmol l
−1
, respectively. With favorable selectivity, stability, reproducibility and ruggedness, the developed MIECS was applied to the determination of Hcy in human serum samples with recoveries of 87.83%–92.58%. The proposed strategy has potential application for disease surveillance. |
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ISSN: | 0013-4651 1945-7111 |
DOI: | 10.1149/1945-7111/ac837b |