Biomarkers of Pulpal Regeneration: Overview on Immunohistochemistry Analysis

Immunocytochemistry (IHC) is a method that uses monoclonal and polyclonal antibodies to determine the tissue distribution of an antigen of interest in health and disease. The method of recognizing a tissue component in situ utilizing unique antibody-antigen interactions in which the antibody is precisely labelled is referred to as IHC. It can be used to identify and localize well-known cellular structures and also extracellular matrix components. This method similarly provides information on the temporospatial distribution of newly discovered carbohydrates and proteins in development, illness and health. Endodontics can use IHC as a diagnostic tool and a potential marker for odontogenic tissues. Although histopathologic examination of periapical lesions reveals the true nature of regenerative tissue, immunohistochemical markers can be utilized to refine the tissue's composition.