Comparison of Antimicrobial and Antipathogenic Efficacy of Tea Tree Oil Nanoemulsion Against Gram Negative Pseudomonas Aeruginosa Infection in Cyprinus Carpio

The major aim of this study is to compare the antimicrobial and antipathogenic efficacy of tea tree oil nanoemulsion against gram negative Pseudomonas aeruginosa and invivo antibacterial efficacy of tea tree oil nanoemulsion in Cyprinus carpio . Materials and methods: Tea tree oil nanoemulsion (TTNE) preparation was done by the standard protocol. Tea tree oil nanoemulsion (N=21) and control antibiotics (Amoxicillin) and zone of inhibition was observed for the well and disc diffusion as per the procedure. Invivo efficacy of tea tree oil nanoemulsion in Cyprinus carpio was performed according to the standard procedure. Results: Independent sample T test was done which revealed that nano formulated tea tree oil showed comparatively good results against Pseudomonas aeruginosa with respect to the control antibiotics. The zone of inhibition in well diffusion was found to be 11 ±1mm for TT oil nanoemulsion and 17.25 ±1mm for antibiotic respectively. Nano formulated tea tree oil appears to have the MIC of 40µl and attained significance was (P