Cryopreservation by Encapsulation-Dehydration of Plumules of Coconut (Cocos nucifera L.)

This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretrea...

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Veröffentlicht in:Cryo-Letters 2008-07, Vol.29 (4), p.339-350
Hauptverfasser: N'Nan, Oulo, Hocher, Valérie, Verdeil, Jean-Luc, Konan, Jean-Louis, Ballo, Koffi, Mondeil, Fanja, Malaurie, Bernard
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Sprache:eng
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Zusammenfassung:This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.
ISSN:0143-2044