Elimination of Precipitates in Oil Red O Fat Stain by Adding Dextrin

Addition of dextrin in the final 60% isopropanol of the Lillie-Ashburu supersaturated oil red O isopropanol technic moderately intensified the stain and decreased our required staining interval. Precipitates were decreased and the diluted solution remained usable into the second week. A saturated 60...

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Veröffentlicht in:Biotechnic & histochemistry 1975-01, Vol.50 (5), p.297-299
Hauptverfasser: Catalano, R. A., Lillie, R. D.
Format: Artikel
Sprache:eng
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Zusammenfassung:Addition of dextrin in the final 60% isopropanol of the Lillie-Ashburu supersaturated oil red O isopropanol technic moderately intensified the stain and decreased our required staining interval. Precipitates were decreased and the diluted solution remained usable into the second week. A saturated 60% isopropanol oil red O solution contained 33 mg/100 ml. Without dextrin the fresh supersaturated solution contains 40 mg, after 3 days 25 mg. With dextrin the fresh solution contained 130 mg dye, the 10-day-old one 100 mg/100 ml.
ISSN:1052-0295
0038-9153
1473-7760
DOI:10.3109/10520297509117078