Staining of Mucus with Buffered Solutions of Toluidine Blue O, Thionin and New Methylene Blue N
The addition of buffer mixtures to toluidine blue O, thionin and new methylene blue N improves their use in the staining of mucus after formaldehyde fixation. Overstaining is minimi The buffer mixtures used consisted of variable proportions of M/10 citric acid and M/5 anhydrous Na2HPO4 in 25% methan...
Gespeichert in:
| Veröffentlicht in: | Biotechnic & histochemistry 1945, Vol.20 (3), p.85-87 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Zusammenfassung: | The addition of buffer mixtures to toluidine blue O, thionin and new methylene blue N improves their use in the staining of mucus after formaldehyde fixation. Overstaining is minimi The buffer mixtures used consisted of variable proportions of M/10 citric acid and M/5 anhydrous Na2HPO4 in 25% methanol. Connective tissue mucus stained satisfactorily with these dyes at a buffer pH range of 3.4 and 3.95 and epithelial mucus at a range of 2.2 to 3.95. The corresponding ratios of M/10 citric acid: M/5 Na2HPO4 are 16:4 to 14:6 and 20 A to 14:6 respectively. |
|---|---|
| ISSN: | 1052-0295 0038-9153 1473-7760 |
| DOI: | 10.3109/10520294509107137 |