A New Method for Safranin Differentiation
A new method of differentiating safranin in pollen-mother-cell smears and paraffin sections is described in detail. Slides stained in safranin are dehydrated in a series of alcoholic solutions containing 1.5% picric acid with constantly decreasing percentages of water. Differentiation is principally...
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| Veröffentlicht in: | Biotechnic & histochemistry 1930, Vol.5 (3), p.103-107 |
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| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | A new method of differentiating safranin in pollen-mother-cell smears and paraffin sections is described in detail. Slides stained in safranin are dehydrated in a series of alcoholic solutions containing 1.5% picric acid with constantly decreasing percentages of water. Differentiation is principally effected in 83% alcohol containing 1.5% picric acid and completed in the final dehydration and clearing. Counterstains may be applied in clove oil if desired. |
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| ISSN: | 1052-0295 0038-9153 1473-7760 |
| DOI: | 10.3109/10520293009115558 |