Prediction of clonogenic cell survival curves based on the number of residual DNA double strand breaks measured by γH2AX staining

Purpose: To assess the potential of using the residual phosphorylation of histone H2AX (γH2AX) after irradiation as a marker of radiosensitivity in vitro. Material and methods: Confluent cell cultures of FaDu and SKX human squamous cell carcinoma lines were irradiated with graded single doses. Twent...

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Veröffentlicht in:International journal of radiation biology 2009-11, Vol.85 (11), p.1032-1041
Hauptverfasser: Menegakis, Apostolos, Yaromina, Ala, Eicheler, Wolfgang, Dörfler, AnnegRet, Beuthien-Baumann, Bettina, Thames, Howard D., Baumann, Michael, Krause, Mechthild
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Sprache:eng
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Zusammenfassung:Purpose: To assess the potential of using the residual phosphorylation of histone H2AX (γH2AX) after irradiation as a marker of radiosensitivity in vitro. Material and methods: Confluent cell cultures of FaDu and SKX human squamous cell carcinoma lines were irradiated with graded single doses. Twenty-four hours after irradiation cells were seeded for standard colony forming assay (CFA). In parallel, staining for γH2AX was performed to visualise the residual foci. Results: In the CFA, FaDu showed a higher radioresistance than SKX. After analysis of the residual foci data, we constructed 'predicted' survival curves using two different methods. First, the proportion of nuclei with
ISSN:0955-3002
1362-3095
DOI:10.3109/09553000903242149