Prediction of clonogenic cell survival curves based on the number of residual DNA double strand breaks measured by γH2AX staining
Purpose: To assess the potential of using the residual phosphorylation of histone H2AX (γH2AX) after irradiation as a marker of radiosensitivity in vitro. Material and methods: Confluent cell cultures of FaDu and SKX human squamous cell carcinoma lines were irradiated with graded single doses. Twent...
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Veröffentlicht in: | International journal of radiation biology 2009-11, Vol.85 (11), p.1032-1041 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Purpose: To assess the potential of using the residual phosphorylation of histone H2AX (γH2AX) after irradiation as a marker of radiosensitivity in vitro.
Material and methods: Confluent cell cultures of FaDu and SKX human squamous cell carcinoma lines were irradiated with graded single doses. Twenty-four hours after irradiation cells were seeded for standard colony forming assay (CFA). In parallel, staining for γH2AX was performed to visualise the residual foci.
Results: In the CFA, FaDu showed a higher radioresistance than SKX. After analysis of the residual foci data, we constructed 'predicted' survival curves using two different methods. First, the proportion of nuclei with |
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ISSN: | 0955-3002 1362-3095 |
DOI: | 10.3109/09553000903242149 |