Application of Nonlinear Regression Analysis to the use of Enzymes in The Determination of Substrate Concentrations

Application of Nonlinear Regression Analysis to the use of Enzymes in The Determination of Substrate Concentrations

A modified nonlinear regression analysis program (originally reported by R.G. Duggleby (1981) Anal. Biochem. 110, 9-18) in BASIC for microcomputers and a modified spectrophotometer are described. Their application in the routine determination of concentrations of certain substances by the use of spe...

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Veröffentlicht in:Instrumentation science & technology 1982-01, Vol.12 (2), p.125-134
Hauptverfasser: Geren, Collis R., Millett, Francis S.
Format: Artikel
Sprache:eng
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Zusammenfassung:A modified nonlinear regression analysis program (originally reported by R.G. Duggleby (1981) Anal. Biochem. 110, 9-18) in BASIC for microcomputers and a modified spectrophotometer are described. Their application in the routine determination of concentrations of certain substances by the use of specific enzymes is detailed. Results are given that show the utility of the system for glucose determination by the hexokinase reaction, and its lack of utility for lactate determination using lactate dehydrogenase.
ISSN:1073-9149
1525-6030
DOI:10.1080/10739148208543552