Molecular methods for the analysis of gut microbiota

Molecular methods for the analysis of gut microbiota

This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Microbial ecology in health and disease 2004-09, Vol.16 (2-3), p.71-85
Hauptverfasser: Namsolleck, Pawel, Thiel, Ralph, Lawson, Paul, Holmstrøm, Kim, Rajilic, Mirjana, Vaughan, Elaine E., Rigottier-Gois, Lionel, Collins, Matthew D., de Vos, Willem M., Blaut, Michael
Format: Artikel
Sprache:eng
Schlagworte:
DGGE
dot blot
FISH
flow cytometry
gut ecology
in situ gene expression
micro-array
sequencing
TGGE
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and flow cytometry is to enumerate faecal bacteria. While flow cytometry is a very fast method, FISH microscopy still has a considerably lower detection limit.
ISSN:0891-060X
1651-2235
1651-2235
DOI:10.1080/08910600410032367