Dual Column HPLC Analysis of Modified Ribonucleosides as Urinary Pathobiochemical Markers in Clinical Research

The quantitative determination of the urinary ribonucleosides m1Ado, m22Guo and t6Ado of breast cancer patients was performed by use of an automated high performance liquid chromatography analyzer with on-line sample processing and analysis by a dual column switching technique. M22Guo and t6Ado showed elevated excretion values (normalized to creatinine) in all of our 24 perioperatively examined breast cancer patients. Studies of these two urinary ribonucleosides 3 years after primary surgery showed markedly reduced excretion values in case that no relapse had occurred.