Normal Phase High Performance Liquid Chromatography of Some Prostaglandin B1 Derivatives

15-Keto-13, 14-trans-prostaglandin B 1 methyl ester, 13,14-trans-prostaglandin B 1 methyl ester, 13,14-cis-prostaglandin B 1 methyl ester, 13,14-dihydro-prostaglandin B 1 methyl ester and 13,14-dehydro-prostaglandin B 1 are organic intermediates used in the synthesis of prostaglandin B x , a polymeric derivative of 15-keto-prostaglandin B 1 methyl ester. PGB x has been shown to protect laboratory animals against cardiogenic shock, cerebral ischemia and hypoxia. A normal phase, high performance liquid chromatographic analysis is presented which permits the identification and quantitation of these PGB 1 intermediates.