Quantitative Determination of the α-Amylase Inhibitor from Phaseolus Vulgaris using size Exclusion High-Performance Liquid Chromatography

A quantitative determination of the α-amylase inhibitor from kidney beans, a glycoprotein (MW 49,000), by the use of a TSK-SW 3000 column is described. A correlation coefficient of 0.986 was obtained when the HPLC method was compared with a biological assay using Beckman Enzymatic Amylase-DS Reagent...

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Veröffentlicht in:	Journal of liquid chromatography 1984-12, Vol.7 (14), p.2895-2903
Hauptverfasser:	Houglum, Joel E., Chappell, Gary S.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha -amylase Analytical, structural and metabolic biochemistry Applied sciences Biological and medical sciences Enzymes and enzyme inhibitors Exact sciences and technology Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology high-performance liquid chromatography Hydrolases Other techniques and industries Phaseolus vulgaris
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Zusammenfassung:	A quantitative determination of the α-amylase inhibitor from kidney beans, a glycoprotein (MW 49,000), by the use of a TSK-SW 3000 column is described. A correlation coefficient of 0.986 was obtained when the HPLC method was compared with a biological assay using Beckman Enzymatic Amylase-DS Reagent Kit. The within-day and between-day coefficients of variation were 4.8-8. 9% and 3.7-11. 6%, respectively. The results indicate this method is a reliable assay that has advantages over the biological assays presently available.
ISSN:	0148-3919 2331-0413
DOI:	10.1080/01483918408067054